ic proinflammatory cytokine and elicits the production of IL-6, whereas IL-6 does not induce IL-1 production. Cytokines Analysis Cytokines were determined by IL-6 and IL-1Ra ELISA kits. The assays employed the quantitative enzyme immunoassay technique. Statistical Analysis Correlations among inflammatory markers and laboratory findings were analyzed by Spearman’s rank correlation coefficient. Repeated measurement ANOVA was conducted within time series between two addicted and non-addicted groups. P values less than 0.05 were considered to indicate statistical significance. Medical Ethics This study was approved by Medical Ethics Council of Shahid Beheshti purchase ABT-267 University of Medical Sciences, Tehran, Iran. Written informed consent was obtained from all participants. Results Cytokines Values of plasma levels of cytokines in opium addicted and nonaddicted 3-vessel disease patients at three times: before, after, and 4 hours after treadmill test are reported in table 1 and figure1. IL-1Ra in the addicted patients was significantly higher than non-addicted patients. Based on repeated measurement PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/22212322 method analysis the between groups test indicates that the variable group is significant and the within subject test indicates that there is not a significant time effect; in other words the groups didn’t change in cytokines over time. IL-6 was not changed significantly over time and was not different between groups. Other Laboratory Tests In the opium addicted patients levels of ESR and LDL-cholesterol 
showed higher median levels that were not statistically significant. There was no significant correlation between laboratory data in the two groups. 3 IL-1Ra Increases in Opium-Addicted CAD Patients Neither IL-1Ra nor IL-6 in itself possesses proinflammatory properties. IL-1Ra production increases under the same inflammatory conditions that stimulate IL-1a and IL-1b. IL-1Ra as an anti-inflammatory mediator most precisely correlated with the occurrence and stage of the disease. Patients with atherosclerosis have higher IL-1Ra level. Antiplatelet therapy which in our study administered to nearly all patients was reported not to cause changes in cytokine antagonist/receptor synthesis. In conclusion our results showed significant high IL-1Ra in the addicted patients compared with non-addicted patients that may be related to harm effects of opium in the CAD patients, and stressor maneuver had no significant effect on this cytokine level. In accordance with other reports, opium not only has any benefit or protective effect in CAD patients, but also is harmful. The authors suggest further study to compare cytokines levels in the addicted and non-addicted healthy persons and also to conduct this study in a larger group to increase the power of the study. Approximately 16% of new HIV diagnoses and two-thirds of new hepatitis C virus diagnoses in the U.S. are in injection drug users . Co-infection among IDUs is common, affecting progression rates and treatment effectiveness for both diseases. During the acute infection phase, standard antibody testing either cannot or has low sensitivity to detect these diseases; however, they can be detected with viral RNA tests. Identification of individuals during this phase of infection may be important in averting infections and improving patient outcomes. The acute phase of HIV infection, lasting approximately 3 months, is characterized by high viral load and high infectivity. The proportion of new infections attributable to in