Maldehyde-fixed cells using the YOL34 monoclonal antibody (1:one hundred; MCA78S AbD Serotec, Raleigh, NC), followed
Maldehyde-fixed cells using the YOL34 monoclonal antibody (1:one hundred; MCA78S AbD Serotec, Raleigh, NC), followed by indirect immunofluorescence applying CY2-conjugated anti-rat antibody (1:100; 31645 Pierce Chemical Co.). Detection of Men…