Ions: H.Z., L.X., X.X and Y.Z. created investigation; H.Z., C.L., L.Z., T.W., D.Z., Y.Z. and Y.L., performed research; H.Z., L.X. and Y.Z. analyzed data; Y.L. and Y.Z. contributed new reagents/analytic tools; H.Z., L.X. and Y.Z. wrote the paper. Funding: This analysis received no Acetylases Inhibitors MedChemExpress external funding. Acknowledgments: The analysis was supported by National Organic Science Foundation of China (No: 31472210). We would like to thank Editage (www.editage.cn) for English language editing. Conflicts of Interest: The authors declare no conflict of interest.
International Journal ofMolecular SciencesArticleLow HIF-1 and low EGFR mRNA Expression Drastically Associate with Poor Survival in Soft Tissue Sarcoma Individuals; the Proteins React DifferentlySwetlana Rot 1 , Helge Taubert two , Matthias Bache 3 , Thomas Greither 4 , Peter W l five , Hans-J gen Holzhausen six , Alexander W. Eckert 1 , Dirk Vordermark three and Matthias Kappler 1, 2 3 4 5Department of Oral and Maxillofacial Plastic Surgery, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany; [email protected] (S.R.); [email protected] (A.W.E.) Clinic of Urology and Pediatric Urology, FA University Hospital Erlangen-Nuremberg, 91054 Erlangen, Germany; [email protected] Department of Radiotherapy, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany; [email protected] (M.B.); [email protected] (D.V.) Center for Reproductive Medicine and Andrology, Martin Luther University, 06120 Halle (Saale), Germany; [email protected] Department of General and Visceral Surgery, Hospital Dessau, 06847 Dessau-Ro au, Germany; [email protected] Institute of Pathology, Martin Luther University Halle-Wittenberg, 06120 Halle (Saale), Germany; [email protected] Correspondence: [email protected]; Tel.: +49-345-557-Received: 30 October 2018; Accepted: 29 November 2018; Published: three DecemberAbstract: In various tumors, the hypoxia inducible factor-1 (HIF-1) along with the epidermal growth factor-receptor (EGFR) have an effect on survival. Nevertheless, the prognostic impact of both markers for soft tissue sarcoma (STS) is not effectively studied. We examined 114 frozen tumor samples from adult soft tissue sarcoma patients and 19 frozen standard tissue samples. The mRNA levels of HIF-1, EGFR, as well as the reference gene hypoxanthine phosphoribosyltransferase (HPRT) had been quantified utilizing a multiplex qPCR approach. Furthermore, levels of EGFR or HIF-1 protein were determined from 74 corresponding protein samples employing ELISA approaches. Our analysis showed that a low degree of HIF-1 or EGFR mRNA (respectively, relative risk (RR) = two.8; p = 0.001 and RR = 1.9; p = 0.04; multivariate Cox regression evaluation) is significantly related using a poor prognosis in STS patients. The combination of both mRNAs inside a multivariate Cox’s regression analysis resulted in an elevated risk of early tumor-specific death of individuals (RR = three.1, p = 0.003) when each mRNA levels within the tumors have been low. The EGFR protein level had no association together with the survival of your patient’s cohort studied, and a higher degree of HIF-1 protein linked only using a trend to significance (multivariate Cox’s regression analysis) to a poor prognosis in STS patients (RR = 1.9, p = 0.09). However, sufferers with low levels of HIF-1 protein and a high content material of EGFR protein in the tumor had a three-fold better survival.