Ulated against the lowest 17)0.003 0.003 0.018 0.018 0.218 0.218 0.463 0.3.4.3. Isolation of Multi-Resistant Staphylococcal Isolates three.four.3. Isolation of Multi-Resistant Staphylococcal Isolates Through the univariable analysis, a significant association with isolation of multiDuring the univariable evaluation, a the bulk-tank milk was evident for four in the resistant staphylococcal isolates fromsignificant association with isolation of multi-resistant staphylococcal isolates in the bulk-tank milk was evident for four in the ten var10 variables evaluated (Table S8). These have been the following: management method apiables evaluated (Tableof lamb removal from their dam,management program appliedmilking, plied within the flock, age S8). These were the following: use of teat disinfection soon after in the flock, length of previous animal farmingdam, use ofof the disinfection soon after milking, and and age of lamb removal from their encounter teat farmer. lengthAmong the variables integrated in the multivariable analysis (Tables S2 and S8), the of preceding animal farming encounter on the farmer. Among the variables incorporated important factor: management program applied in the following emerged to become the only inside the multivariable analysis (Tables S2 and S8), the following=emerged to be four and Table 4). flock (p 0.015) (Figure the only important factor: management program applied within the flock (p = 0.015) (Figure four and Table 4).Biology 2021, ten, 1016 Biology 2021, 10,9 of 15 9 ofFigure 4. Proportion of flocks from which multi-resistant staphylococcal isolates were recovered, in recovered, Figure 4. Proportion of flocks from in accordance with all the management technique applied in these flocks (Ro 5212773 Antagonist dashed line: trendline). accordance together with the management technique applied in these flocks (dashed line: trendline). Table four. Outcomes of multivariable evaluation for isolation of multi-resistant staphylococcal isolates from Table four. Results of multivariable analysis for isolation of multi-resistant staphylococcal isolates from the bulk-tank milk of 325 sheep flocks in Greece. the bulk-tank milk of 325 sheep flocks in Greece.Variable (n = 1)Variable (n = 1)Odds Ratios 1 (95 Self-assurance Intervals) (95 Self-assurance Intervals)Odds RatiosppManagement technique applied inside the flock Management system applied within the flock Intensive = = 43) Intensive (n(n 43) Semi-intensive (n 151) Semi-intensive (n = = 151) Semi-extensive (n(n 107) Semi-extensive = = 107) In depth (n(n 24) Extensive = = 24)13.939 (1.74511.329) 13.939 (1.74511.329) three.512 (0.4497.460) three.512 (0.4497.460) 1.859 (0.2215.606) 1.859 (0.2215.606) Reference Reference0.015 0.013 0.013 0.23 0.23 0.57 0.0.Odds ratios calculated against lowest prevalence associations of the variables. Odds ratios calculated against the the lowest prevalence associations with the variables.four. Discussion 4. Discussion A scientific opinion of the European Meals Security Authority [19] pointed the the A scientific opinion in the European Food Security Authority [19] pointed out out imimportance antibiotic resistance of of bacteria isolated from raw milk forthe public health. portance of of antibiotic resistance bacteria isolated from raw milk for the public well being. Therefore, there is a scope to study the patterns of such resistance in sheep farms. Additionally, Hence, there’s a scope to study the patterns of such resistance in sheep farms. Moreover, the evaluation and identification of predictors associated with the management inside the farms the evaluation and identification of pred.