In G. sinensis under Salt Pressure plus the Effect of Exogenou 0.05.Agriculture 2021, 11, x FOR PEER Critique Agriculture 2021, 11,11 of 21 10 ofFigure five. Effects of different salt concentrations and addition of exogenous calcium ions around the Figure 5. Effects+of various salt concentrations and addition of exogenous calcium ions on the contents of Na , K+ , and Ca2+ in G. sinensis seedlings. (A ) Na+ , K+ , and Ca2+ content in differcontents of Na+, K+, and Ca2+ in G. sinensis seedlings. (A ) Na+, K+, and Ca2+ content in distinctive ent tissue parts of G. sinensis at unique salt concentrations and with distinctive concentrations of tissue components of G. sinensis at different salt concentrations and with different concentrations of calcalcium CK is actually a G. sinensis plant plant in standard hydroponic culture in Hogland Hogland nutrient cium ions.ions. CK is often a G. sinensisgrown grown in normal hydroponic culture in nutrient solusolution. S1 and G. sinensis plants treated with concentration of 100 of 100 mmol/L NaCl, retion. S1 and S2 areS2 are G. sinensis plants treated with concentrationand 200and 200 mmol/L NaCl, spectively; S1+C1, + C1, S1 + C2, andrepresent represent one hundred mmol/L the additionthe five, 10, and of five, 10, respectively; S1 S1+C2, and S1+C3 S1 + C3 one hundred mmol/L NaCl with NaCl with of addition 15 mmol/LCaCl2, respectively. The ion content material ion content was measured and analyzed by inductively and 15 mmol/LCaCl2 , respectively. The was measured and analyzed by inductively coupled plasma optical emission spectrometry soon after a single week ofone week of plant therapy. Theaverage the coupled plasma optical emission spectrometry soon after plant therapy. The data would be the information are SD of three independent replicates. The mean values represented by the distinct letters had been sigaverage SD of 3 independent replicates. The mean values represented by the distinctive letters nificantly various in Tukey’s test at p 0.05. were drastically distinct in Tukey’s test at p 0.05.3.ten. Effects of of Exogenous Calcium on Plant Phenolic Compounds below Salt Anxiety three.ten. Effects Exogenous Calcium on Plant Phenolic Compounds below Salt Pressure Subsequently, we studied the accumulation of phenolic compounds in in distinct Subsequently, we studied the accumulation of phenolic compounds different tissues tissues treated with one hundred mmol/L NaCl (S1), 100 NaCl + 10 mmol/L CaCl (S1 + C2), and treated with one hundred mmol/L NaCl (S1), 100 mmol/L mmol/L NaCl + ten mmol/L CaClnormal growth state (CK). Visualization of phenolic supplies was performed by hierarchical cluster evaluation (Figure 6). Twenty-three phenolic compounds and L-phenylalanine had been determined by the LC S method in Levamlodipine besylate Autophagy methanol extracts of roots, stems, and leavesAgriculture 2021, 11,11 ofof G. sinensis, plus the forms of compounds measured for the differently treated samples showed no difference. It may be seen in the heat map that these compounds have been mainly divided into three groups around the basis of their tissue-specific accumulation patterns and participated in salt stress responses to varying degrees. The C6C3-type SJ995973 Cancer compound (p-hydroxycinnamic acid, p-coumaric acid), the C6C3C6-type compound (naringenin, glycyrrhizin, genistein, quercetin), and L-phenylalanine have been mainly accumulated within the roots (Figure 7A). There was a slight boost but not important in the case of salt therapy alone, and a very substantial increase in case of exogenous calcium addition. The C6C1type compound (two,5-dihydroxybenzoic acid) plus the.