Te (Figure 4I). These data look to suggest a decrease amount
Te (Figure 4I). These data seem to suggest a reduce amount despite the preferential growth of preferential development of neurons on To much better comprehend To greater comprehend the theneurons on the graphene substrate.the graphene substrate.the molecular mechanism behind the preferential adhesion of neurons on adhesion of neurons on a given substrate, molecular mechanism behind the preferential a provided substrate, we measured the number two of measured the amount of In this case, we per 2 Within this case, we observed a sigwevinculin Niacin-13C6 Endogenous Metabolite clusters per . vinculin clustersobserved. a considerably higher quantity of vinculin larger when cells are grown on graphene (Figure 4J). This result suggests a nificantlyclusters quantity of vinculin clusters when cells are grown on graphene (Figure vinculin result suggests a vinculin reorganization driven highly fragmented vinculin 4J). This reorganization driven by the substrate, displaying aby the substrate, displaying a distribution on graphene. highly fragmented vinculin distribution on graphene.Figure four. Quantitative super-resolution imaging of vinculin in neurons (DIV3). STORM imaging (A) and cluster evaluation (B) Figure four. Quantitative super-resolution imaging of vinculin in neurons (DIV3). STORM imaging (A) and cluster analysis of vinculin immuno-stained with A405/A647 in neurons seeded on glass. Frequency distribution of localizations/cluster (B) of vinculin immuno-stained with A405/A647 in neurons seeded on glass. Frequency distribution of localizaand cluster region measured in cells seeded on glass (C). STORM imaging (D) and clustering (E) of vinculin immuno-stained tions/cluster and cluster region measured in cells seeded on glass (C). STORM imaging (D) and clustering (E) of vinculin with A405/A647 in neurons seeded on SLG. Frequency distribution of localizations and cluster region in cells cluster on SLG immuno-stained with A405/A647 in neurons seeded on SLG. Frequency distribution of localizations and grown region in (F). grown on SLG distribution shows distribution shows 6 reduce in of Tetraphenylporphyrin MedChemExpress typical events of for vinculin in (G) cellsThe cumulative (F). The cumulative a six reduce in theaaverage numberthe localizednumber(G)localized eventscells adhered on SLG (61.two 1.two N SLG (61.two 1.2 Nloc/cluster) compared on glass (65.1 2.7 Nloc /cluster). The cumulative for vinculin in cells adhered onloc /cluster) in comparison to cells adheredto cells adhered on glass (65.1 two.7 Nloc/cluster). The distribution shows a six increase in boost in the typical cluster area (H) in on adhered on SLG two ) compared ) cells cumulative distribution shows a six the average cluster area (H) in cells adheredcellsSLG (943 10 nm(943 ten nm2tocompared to on glass (890 19glass). The cumulative distribution shows a 23 reduce a 23 reduce in typical density (I) on adhered cells adhered on nm2 (890 19 nm2). The cumulative distribution shows in typical density (I) in cells adhered in 2 cells (0.072 0.002 Nloc(0.072 ) ompared to cellscompared to cells adhered on glass/nm2 ). The average/nm2). The vinculin SLG adhered on SLG /nm2 0.002 Nloc/nm ) adhered on glass (0.093 0.005 Nloc (0.093 0.005 Nloc number of average 2) in comparison with number of vinculin clusters/area (J) increasesgraphene (21.0 on4 N two clusters/area (J) increases in cells grown on in cells grown graphene (21.0 four Ncluster/ glass (eight.0 0.9 glass (eight.0 0.9 Ncluster / two ). cluster / ) in comparison with Ncluster/ 2). The box shows 25/75th percentiles plus the whiskers will be the normal deviation, T-test statistic.