Exosomes by immunofluorescence. Kinetics of mRNA expression of Th1 transcription components (T-bet, STAT-1, and STAT-4) by RT-PCR and IL-2/IFN- quantification by ELISA. Results: DENV-2 had a titer of 1.0 107PFU/mL. Infection brought on morphological modifications in MO for instance filipodia formation and surface adhesions. Exosome concentration was 7.0 1010 particles/mL in two.0 107 infected MO. A significant difference (p 0.05) was obtained in concentration between infected and mock MO. All fractions on the gradient have been enriched with CD63+ exosomes. TEM showed an exosome heterogeneous (shape and size) population. The rest on the experimental approach is under development. Results: Within this study, we’re evaluating the function of tetraspanin CD63 in exosomes as co-stimulatory molecule in an in vitro model of DENV infection.PT11.RSV site Uncovering the immunomodulatory possible of mesenchymal stromal cells-derived extracellular vesicles Raquel Cunha1, Alisa Ugodnikov1, Thomas Kuncewicz1, Helena Lan1, Heidi Kuang1, Kelvin Ng1, Oren Levy1,two, Rachelle Prantil-Baun2, Cl dia Lobato da Silva3, Joaquim Cabral3, Jeffrey Karp1 and Donald Ingber1 Brigham and Women’s Hospital, Harvard Healthcare School, MA, USA; 2Wyss Institute for Biologically Inspired Engineering at Harvard University, MA, USA; 3Department of Bioengineering and iBB-Institute for Bioengineering and Biosciences, Instituto Superior T nico, Universidade de Lisboa, Lisbon, PortugalExosomes are tiny secreted membrane vesicles located in tissues, synovial fluids and all other compartments within the body. Intercellular messengers, cargo delivery of effector or signalling macromolecules, and cytokine communication among certain cells are a number of in the critical functions ascribed to exosome to date. Exosomes happen to be reported in a quantity of acute inflammatory processes in illness or by environmental causes. In this report we investigate the exosome formation and cargo content material for the duration of inflammation in Factor Xa site lipopolysaccharide stressed human A549 human lung epithelial cells as well as the restoration impact of inflammation and oxidative strain by near-infrared photobiomodulation, as a brand new therapeutic method in pulmonary inflammation model.PT11.Tetraspanin CD63 in exosomes derived from human monocytes participates as co-stimulatory molecule within the immunological synapse during dengue virus infection Pedro Pablo Mart ez Rojas, Ver ica Monroy-Mart ez and Blanca RuizOrdaz Biomedical Analysis Institute, National Autonomous University of Mexico, Mexico City, MexicoIntroduction: Dengue fever (DF) is the most important arthropod-borne viral illness in tropical areas. Dengue virus (DENV) infection impacts much more than 100 million people today worldwide each and every year. DF is brought on by any in the 4 serotypes of DENV and presents a broad clinical spectrum, ranging from a benign self-limiting infection for the severe dengue (SD). DENV primarily infects antigen presenting cells (APC) and monocytes (MO). The contact area between APC and T cells (TC) is known as immunological synapse (IS). Tetraspanins are integrins that function as costimulatory molecules in the course of TC activation. Tetraspanin CD63 is abundantly in endosomes of APC and MO. The part of tetraspanin CD63 in exosomes, in the absence of CD80/CD86, on the elevated TC activity observed in DF continues to be not described and could possibly be vital for the cytokine storm reported in SD instances. Approaches: DENV-2 amplification in C6/36 cells and viral titration by lytic plate assay. Cell culture of THP-1 ATCC TIB-202 (MO) and J.