nificant distinction was observed in between the CT and RU group for all these fertility parameters.Toxics 2021, 9,oestradiol concentrations enhanced in RU as in comparison with CT animals at Day 36 but not at Day 50 (Figure S4). Additionally, at Day 36, the protein degree of the cholesterol side-chain cleavage enzyme (P450scc) plus the cholesterol level within the testes was greater in RU animals that in control animals, whereas the 3-beta ydroxysteroid dehydrogenase (3HSD) level and the quantity of your cholesterol carrier, steroidogenic acute regulatory protein (STAR), was similar in both groups (Figure S4). This positive effect of dietary RU exposure was no longer observed at D50 (Figure S4).13 ofFigure 5. Plasma testosterone, oestradiol and chemerin concentrations in CT and RU animals. (A) Concentration of blood plasma testosterone (ng/mL). Stars () correspond to the unpaired t-test significance (p 0.05) amongst CT and RU rooster groups at diverse occasions. (B) Concentration of plasma oestradiol (pg/mL). Stars () correspond H1 Receptor Inhibitor site towards the unpaired t-test significance (p 0.05) amongst CT and RU rooster groups at unique times. p 0.01. (C) Concentration of plasma chemerin (ng/mL). Stars () correspond towards the unpaired t-test significance (p 0.01) between CT and RU rooster groups at different times. p 0.01; p 0.0001.Toxics 2021, 9, 318 021, 9, x FOR PEER REVIEW15 of14 ofFigure 6. Consequences of RU exposure on the metabolism of offspring. (A) Evaluation of RU and CT chicks’ food Figure 6. Consequences of RU exposure on the metabolism of offspring. (A) Analysis of determined. Stars () consumption at postnatal Days (PND) 5 and ten. Average meals consumption per pen (g/day) was RU and CT chicks’ meals consumption at postnatal Days (PND) 5 and 10. Typical meals consumption per correspond for the unpaired t-test significance (p 0.05). (B) Physique weight (g) of RU (n = 118) and CT chicks (n = 109) at birth pen (g/day) was determined. Stars () correspond for the unpaired t-test significance (p 0.05). (B) and on PND five and ten (g). Stars () correspond towards the unpaired t-test significance (p 0.05). p 0.0001. (C) Typical Body weight (g) of RU (n = 118) and CT chicks (n = 109) at birth and on PND 5 and 10 (g). Stars () day-to-day achieve (g/day) for CT the= 109) andt-test significance (p 0.05). and 0.0001. (C) Typical dailyto the unpaired t-test correspond to (n unpaired RU chicks (n = 118) on PND five p 10. Stars () correspond obtain significance (p 0.01), CT (n 0.001. (D)RU chicks (nof 118) ratio in between the digestive correspond to thethe physique weight (g/day) for p = 109) and Evaluation = the on PND five and ten. Stars () tract weight and unof CT chicks (n = 10) and RU chicks (n = 0.01), p and on(D) Evaluation ( ). Stars () corresponddigestive paired t-test significance (p ten) at birth 0.001. PND five and ten from the ratio among the for the unpaired t-test significance (p weight(E) Evaluationweight ratio among Bcl-xL Inhibitor custom synthesis subcutaneous chicks (ntissueat birth and on PND tract 0.05). and also the physique from the of CT chicks (n = 10) and RU adipose = ten) weight and body weight of CT five and ten ( ). Stars () correspond chicks (n = ten, 5 males and five females) 0.05). and on PND five and ten. chicks (n = 10, five males and 5 females) and RU for the unpaired t-test significance (p at birth(E) Evaluation from the Stars () ratio between subcutaneous adipose 0.01). correspond to the unpaired t-test significance (p tissue weight and body weight of CT chicks (n = ten, five males and 5 females) and RU chicks (n = 10, 5 males and five fema