The root nodule-enhanced transcriptome in soybean. Mol Cells 2004, 18(1):532. six. Oh CJ, Lee H, Kim HB, An CS: Isolation and characterization of a root nodule-specific cysteine proteinase cDNA from soybean. J Plant Biol 2004, 47(3):21620. 7. Espinosa-Victoria D, Vance CP, Graham PH: Host variation in traits linked to crown nodule senescence in soybean. Crop Sci 2000, 40(1):10309. 8. Pfeiffer NE, Torres CM, Wagner FW: Proteolytic activity in soybean root nodules: activity in host cell cytosol and bacteroids all through physiological development and senescence. Plant Physiol 1983, 71(4):79702. 9. Sheokand S, Dahiya P, Vincent JL, Brewin NJ: Modified FP Antagonist Compound expression of cysteine protease affects seed germination, vegetative development and nodule development in transgenic lines of Medicago truncatula. Plant Sci 2005, 169(5):96675. 10. Li Y, Zhou L, Chen D, Tan X, Lei L, Zhou J: A nodule-specific plant cysteine proteinase, AsNODF32, is involved in nodule senescence and nitrogen fixation activity from the green manure legume Astragalus sinicus. New Phytol 2008, 180(1):18592. 11. Alesandrini F, Frendo P, Puppo A, H ouart D: Isolation of a molecular marker of soybean nodule senescence. Plant Physiol Biochem 2003, 41(eight):72732. 12. Vorster B, Schl er U, du Plessis M, van Wyk S, Makgopa M, Ncube I, Quain M, Kunert K, Foyer C: The cysteine protease ysteine protease inhibitor technique explored in soybean nodule improvement. Agron 2013, three(3):55070. 13. BChE Inhibitor supplier Tajima T, Yamaguchi A, Matsushima S, Satoh M, Hayasaka S, Yoshimatsu K, Shioi Y: Biochemical and molecular characterization of senescence-related cysteine protease ystatin complex from spinach leaf. Physiol Plant 2011, 141(two):9716. 14. Pillay P, Schluter U, van Wyk S, Kunert KJ, Vorster BJ: Proteolysis of recombinant proteins in bioengineered plant cells. Biogeosciences 2014, five(1):150. 15. Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, Might GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, et al: Genome sequence on the palaeopolyploid soybean. Nature 2010, 463(7278):17883. 16. Severin AJ, Woody JL, Bolon YT, Joseph B, Diers BW, Farmer AD, Muehlbauer GJ, Nelson RT, Grant D, Specht JE, Graham MA, Cannon SB, May possibly GD, Vance CP, Shoemaker RC: RNA-Seq Atlas of Glycine max: a guide for the soybean transcriptome. BMC Plant Biol 2010, ten(1):160. 17. McIntyre LM, Lopiano KK, Morse AM, Amin V, Oberg AL, Young LJ, Nuzhdin SV: RNA-seq: technical variability and sampling. BMC Genomics 2011, 12:293. 18. Hara-Nishimura I, Hatsugai N, Nakaune S, Kuroyanagi M, Nishimura M: Vacuolar processing enzyme: an executor of plant cell death. Curr Opin Plant Biol 2005, eight(four):40408. 19. Roberts IN, Caputo C, Criado MV, Funk C: Senescence-associated proteases in plants. Physiol Plant 2012, 145(1):13039. 20. Martinez M, Cambra I, Carrillo L, Diaz-Mendoza M, Diaz I: Characterization from the whole cystatin gene family members in barley and their target Cathepsinvan Wyk et al. BMC Plant Biology 2014, 14:294 http://biomedcentral/1471-2229/14/Page 13 of21.22.23.24.25.26.27. 28.29.30.31.32.33.34.35. 36.37.38.39.L-like cysteine-proteases, partners in the hordein mobilization through seed germination. Plant Physiol 2009, 151(three):1531545. Richau KH, Kaschani F, Verdoes M, Pansuriya TC, Niessen S, St er K, Colby T, Overkleeft HS, Bogyo M, Van der Hoorn RAL: Subcla.