Product Name :
LC3 monoclonal antibody (5H3)
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Stable at -20°C up to 1 year.
Description:
Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.{{2553413-93-5} site|{2553413-93-5} Technical Information|{2553413-93-5} Data Sheet|{2553413-93-5} custom synthesis}
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| Alternative Name Microtubule-associated protein 1 light chain 3, MAP1LC3 | Application ICC, IHC, WB | Application Notes Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot. | Clone 5H3 | Formulation Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose. | Host Mouse | Immunogen Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin.{{1802148-05-5} MedChemExpress|{1802148-05-5} Technical Information|{1802148-05-5} In Vivo|{1802148-05-5} supplier} | Isotype IgG1 | Positive Control Included.PMID:30725717 | Recommendation Dilutions/Conditions Immunocytochemistry (paraformaldehyde/methanol fixation; 1-10 µg/ml)Western Blot (0.5µg/ml for HRPO/ECL detection; Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)We strongly recommend to use PVDF membranes for immunoblot assays.Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Human | Specificity Recognizes both forms of endogenous LC3. | UniProt ID Q9H492 (LC3A), Q9GZQ8 (LC3B), Q9BXW4 (LC3C) | Unit of Measure (UM) µg