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[pSer55]Vimentin monoclonal antibody (4A4)
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Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell. Western blot analysis of U251 interphase (1) and U251 M phase (2) cells, probed with Vimentin (pSer55) mAb (4A4). Immunocytochemistry analysis of U251 cells using Vimentin (pSer55) mAb (4A4). Western blot analysis of U251 interphase (1) and U251 M phase (2) cells, probed with Vimentin (pSer55) mAb (4A4). Immunocytochemistry analysis of U251 cells using Vimentin (pSer55) mAb (4A4).{{4474-91-3} web|{4474-91-3} Protocol|{4474-91-3} In stock|{4474-91-3} manufacturer}
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| Alternative Name VIM | Application ELISA, ICC, WB | Application Notes Detects a band of ~57kDa by Western blot.{{474645-27-7} medchemexpress|{474645-27-7} Technical Information|{474645-27-7} In Vivo|{474645-27-7} supplier} | Clone 4A4 | Formulation Liquid.PMID:29083576 In PBS containing 50% glycerol. | Host Mouse | Immunogen Synthetic peptide corresponding to a portion of mouse vimentin phosphorylated at Ser55. | Isotype IgG2b | Recommendation Dilutions/Conditions ELISA, Immunocytochemistry (1µg/ml)Western Blot (1-5µg/ml)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Human, Mouse, Rat | UniProt ID P20152 | Unit of Measure (UM) µg