Ozos Received: 15 September 2021 Accepted: 8 October 2021 Published: 9 October5Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece; [email protected] (D.T.L.); [email protected] (P.J.C.); [email protected] (D.A.G.); [email protected] (C.K.M.); [email protected] (V.S.M.) University Hospital of Larissa, 41110 Larissa, Greece; [email protected] (E.P.); [email protected] (K.T.); [email protected] (A.S.) Faculty of Public and 1 Health, University of Thessaly, 43100 Karditsa, Greece; [email protected] Faculty of Animal Science, University of Thessaly, 41110 Larissa, Greece; [email protected] (N.G.C.V.); [email protected] (T.G.) Division of Agriculture, University of Ioannina, 47132 Arta, Greece; [email protected] Division of Agriculture, Food, Organic Resources and Engineering (DAFNE), University of Foggia, 71122 Foggia, Italy; [email protected] Correspondence: [email protected] Summary: This operate investigated the presence of staphylococci resistant to antibiotics in the raw milk produced in sheep farms, that is intended for human consumption, in an extensive study performed throughout Greece. In 31 of flocks, staphylococci resistant to at the very least 1 antibiotic had been recovered; in 12 of flocks, staphylococci resistant to no less than three Buprofezin manufacturer isolates had been the lack of expertise by farmers (5 years), the period instantly post lambing, along with the intensive management technique applied within the flocks. Abstract: The objectives of this work were to study prevalence and characteristics of resistance to antibiotics of staphylococcal isolates from the bulk-tank milk of sheep flocks across Greece, to assess feasible associations of your presence of antibiotic resistance with the high quality of milk in these flocks and to evaluate flock-related aspects potentially associated with antibiotic resistance amongst these isolates. A cross-sectional study was performed in 325 sheep flocks in Greece. Bulk-tank milk samples were collected for bacteriological examination; staphylococcal isolates were evaluated for resistance to 20 antibiotics. Oxacillin-resistant staphylococcal isolates, isolates resistant to any antibiotic, and multi-resistant isolates were recovered from eight.0 , 30.5 , and 12.0 of flocks, respectively. Of 232 isolates, 11.6 had been resistant to oxacillin, 46.1 had been resistant to a minimum of one antibiotic, and 16.4 were multi-resistant. Resistance was seen much more often among coagulase-negative (50.6 ) than amongst Staphylococcus aureus (31.five ) isolates. Resistance was a lot more frequent against penicillin and ampicillin (34.1 of isolates), clindamycin (17.7 ), and fosfomycin (14.two ). An association was located among biofilm formation by staphylococci and resistance to fosfomycin. For recovery of oxacillin-resistant isolates, the lack of knowledge by farmers emerged as a substantial issue; respective elements for the isolation of staphylococci resistant to any antibiotic or multi-resistant isolates had been the early stage from the lactation period (0thst month) and the intensive management system applied within the flocks, respectively. Key phrases: bulk-tank milk; mastitis; methicillin; milk; sheep; somatic cell counts; staphylococcus; tetracycline; total bacterial countsPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the author.