On compared with cold-acclimated plants. The lower was most drastic in Aydanhanim (Figure 7). Changes in guaiacol peroxidase activity triggered by de-acclimation showed distinct patterns amongst the barley accessions (Figure 7). In Aday-4, DS1028, and Carola, activity was reduce during and after de-acclimation compared with that CB1 Antagonist Species recorded for cold-acclimated plants. In DS1028 and Carola, activity rose at DA-28 compared with that at DA-23, but didn’t attain the level of activity observed after cold acclimation (CA-21). In Astartis and Mellori, a slight lower in guaiacol peroxidase activity was observed at the beginning of de-acclimation but was followed by a considerable increase after one particular week of deacclimation, attaining greater activity than that observed in cold-acclimated plants. In Aydanhanim, DS1022, and Pamina, the guaiacol peroxidase activity was higher in the course of (DA-23) and immediately after (DA-28) de-acclimation than after cold acclimation (CA-21). In Calcium Channel Inhibitor Synonyms DS1022 and Pamina, the activities recorded in the DA-23 and DA-28 time points have been equivalent,Int. J. Mol. Sci. 2021, 22,22 ofwhereas in Aydanhanim, the guaiacol peroxidase activity at DA-28 was distinctly decrease than that at DA-23 (Figure 7).Figure 7. Modifications in antioxidant activity of peroxidases: Ascorbate, glutathione, guaiacol, and nonspecific peroxidase in six time points–before cold acclimation (CA-0 (C)), during acclimation to cold (CA-7), after 3-week cold acclimation (CA-21), through de-acclimation (DA-23), following 7-day de-acclimation (DA-28), and during re-acclimation to cold (RA-35) in tolerant (left) and susceptible (appropriate) to de-acclimation barley accessions. The de-acclimation period is indicated between the vertical dashed lines.The pattern of nonspecific peroxidase activity differed amongst all the tested barley accessions, but some similarities had been observed (Figure 7). The activity enhanced initially through de-acclimation in DS1028 and Pamina, then decreased to a level similar to thatInt. J. Mol. Sci. 2021, 22,23 ofrecorded for cold-acclimated plants soon after seven days of de-acclimation. The profile of adjustments triggered by de-acclimation was related for Aydanhanim, however the reduce at DA-28 was smaller sized, but the activity remained greater at DA-28 than in CA-21. In Mellori nonspecific peroxidase activity progressively improved owing to de-acclimation and decreased quickly during re-acclimation to cold. In Carola and DS1022, the initial decrease in nonspecific peroxidase activity observed at DA-23 was followed by a rapid improve at DA-28, resulting in greater activity than that recorded in CA-21. In Aday-4 a decrease in nonspecific peroxidase activity through and after de-acclimation was observed. No adjustments in nonspecific peroxidase activity triggered by de-acclimation have been observed for Astartis (Figure 7). The profile of changes in formate dehydrogenase activity caused by de-acclimation was comparable for five barley accessions (Figure eight). In Astartis, Aydanhanim, Carola, DS1028, and Pamina, activity enhanced significantly within the initial stage of de-acclimation (DA-23) and decreased rapidly following seven days of de-acclimation. The reduce led to activity lower than that observed in CA-21 in four on the accessions. In Aday-4 and Mellori, the formate dehydrogenase activity was reduced during and right after de-acclimation compared with that of cold-acclimated plants. The activity remained low also during re-acclimation to cold. In DS1022, formate dehydrogenase activity enhanced throughout and immediately after de-acclim.