nuscript.Funding: The authors are very grateful for the Workplace of Research Help at Qatar University (grant (grant Funding: The authors are extremely grateful for the Office of Investigation Help at Qatar University no. QUCGCPH20/211) as well as the Qatar Foundation–Qatar National Analysis Fund (UREP27160 no. QUCG-CPH-20/21-1) and the Qatar Foundation–Qatar National Research Fund (UREP27-160-33045), to H.M.K., for funding this function. 045), to H.M.K., for funding this work. Acknowledgments: Figures 1 and 3 were generated making use of publicly readily available tools from Servier Servier Acknowledgments: Figures 1 and 3 have been generated working with publicly offered tools from Medical Art (Clever), which are licensed under a Creative Commons Attribution 3.0 Unported License Medical Art (Wise), that happen to be licensed beneath a Creative Commons Attribution 3.0 Unported License and vectors from Freepik (freepik, accessed on 16 June 2021).and vectors from Freepik (freepik, accessed on 16 June 2021). Conflicts of Interest: The authors declare no conflict of interest.Conflicts of Interest: The authors declare no conflict of interest.
International Journal ofMolecular SciencesArticleComparing Early Transcriptomic Responses of 18 Soybean (Glycine max) Genotypes to Iron StressDaniel R. Kohlhase 1 , Chantal E. McCabe two, , Asheesh K. Singh 1 , Jamie A. O’Rourke two, and Michelle A. Graham two, Department of Agronomy, Iowa State University, Ames, IA 50011, USA; [email protected] (D.R.K.); [email protected] (A.K.S.) U.S. Department of Agriculture (USDA)–Agricultural Research Service (ARS), Corn Insects and Crop Genetics Research Unit, Ames, IA 50011, USA; [email protected] Correspondence: [email protected] (J.A.O.); [email protected] (M.A.G.) Present address: Division of Quantitative Wellness Sciences, Mayo Clinic, Rochester, MN 55901, USA.Citation: Kohlhase, D.R.; McCabe, C.E.; Singh, A.K.; O’Rourke, J.A.; Graham, M.A. Comparing Early Transcriptomic Responses of 18 Soybean (Glycine max) Genotypes to Iron Anxiety. Int. J. Mol. Sci. 2021, 22, 11643. doi.org/10.3390/ ijms222111643 Academic Editor: Anna M. Mastrangelo Received: 23 September 2021 Accepted: 25 October 2021 Published: 28 OctoberAbstract: Iron deficiency chlorosis (IDC) is an abiotic stress that negatively affects soybean (Glycine max [L.] Merr.) production. Much of our knowledge of IDC strain responses is derived from model plant species. Gene expression, quantitative trait loci (QTL) mapping, and genome-wide association research (GWAS) performed in soybean recommend that anxiety response variations exist amongst model and crop species. Our existing understanding of the molecular response to IDC in soybeans is largely derived from gene expression studies applying near-isogenic lines differing in iron efficiency. To improve iron efficiency in soybeans and also other crops, we ought to expand gene expression studies to consist of the diversity present in germplasm collections. Hence, we collected 216 purified RNA samples (18 genotypes, two tissue kinds [leaves and roots], two iron treatments [sufficient and deficient], 3 replicates) and utilized RNA sequencing to examine the expression differences of 18 diverse soybean genotypes in response to iron deficiency. We found a rapid response to iron deficiency across genotypes, most responding inside 60 min of pressure. There was little proof of an overlap of precise differentially expressed genes, and comparisons of gene ontology terms and CDC Inhibitor Compound transcription CB2 Antagonist Gene ID factor famil