Product Name :
Anti-Rabbit IgG kappa, Goat antibody(Biotin)
Applications:
WB,ELISA,IP
Reactivity :
Rabbit IgG kappa
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-Rabbit IgG kappa, Goat antibody(Biotin) is designed for detecting rabbit IgG kappa chain specifically. Anti-Rabbit IgG kappa, Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to rabbit IgG kappa chain coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG kappa, Goat antibody(Biotin) reacts with rabbit IgG kappa chain selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG. | Immunogen: Recombinant Rabbit IgG | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation) | Conjugate: Biotin-SP (long spacer) | Specificity: Rabbit IgG kappa chain | Cross-Reactivity: No cross-reactivity with mouse, human, cynomolgus, rat, goat IgG | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS(pH 7.{{115819-92-6} medchemexpress|{115819-92-6} Protocol|{115819-92-6} In stock|{115819-92-6} supplier} 5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C | Background:Rabbit research antibodies are widely used in life science research. So far, four isotypes have been identified (IgA, IgE, IgG, and IgM) in rabbits. Each isotype has a different heavy chain. Rabbit has only one IgG subclass. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains.{{1254053-43-4} MedChemExpress|{1254053-43-4} Technical Information|{1254053-43-4} Description|{1254053-43-4} custom synthesis} The heavy chain is about 50 KD and the light chain is about 25 KD.PMID:30236515 The common IgG is monomeric with a molecular weight of approximately 150 kD.Using antibody with Fc(mutation), the background from Fc receptors will be eliminated.
Description2 :
Anti-Rabbit IgG kappa, Goat antibody(Biotin) is designed for detecting rabbit IgG kappa chain specifically. Anti-Rabbit IgG kappa, Goat antibody(Biotin) is based on monoclonal, recombinant, goat IgG Fc fused single domain antibody to rabbit IgG kappa chain coupled to Biotin. Based on immunoelectrophoresis and/or ELISA, Anti-Rabbit IgG kappa, Goat antibody(Biotin) reacts with rabbit IgG kappa chain selectively, no reactivity with mouse, human, cynomolgus, rat, goat IgG.
Immunogen:
Recombinant Rabbit IgG
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c fused to goat IgG Fc(mutation)
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), Stable for 12 months at -20°C
Function:
ELISA：1:10000-1:50000WB: 1:10000-1:50000 IP: 1-2ug/sampleDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.