structural protein Gag can be initiated both through the canonical cap-dependent mechanism, or by the alternative IRES-dependent mechanism. The capped and polyadenylated HIV-1 genomic RNA has been described to harbor…
turer's protocol. Briefly, 300 ml of a 0.56106 cells per milliliter solution were plated in serum-free RPMI-1640 in the upper chamber of an insert. The bottom portion of the well…
the first and second experiment, respectively. To find out if hrpL expression itself is linearly dependent on the bacterial cell number, normalized hrpL expression was compared to the absolute copy…
RNA as in. Two passages of the 5538, 7711 and 0029 fibroblasts were similarly treated to extract and amplify RNA. In situ Hybridization Whole-mount in situ hybridization. The isolated embryonic…
optosis, tumour immunogenicity and cancer cell death. Consistent with these finding, CDV and MV infections showed promising oncolytic activity in a mouse model of lymphoma carcinomas. The MV-mediated
t birth. Consistent with our in situ hybridization results, we observed robust expression of Runx2 in both the vertebral body and the adjacent ligament. To confirm that Runx2 is expressed…
mplified by PCR with primers that included a BamHI site at the 59 end and a NotI site at the 39 end of the A2 domain. The amplified fragment was…
estimated using sandwich ELISA. denaturation at 95uC for 15 s; step 3-annealing at 57uC for 15 s; step 4-extension at 72uC for 20 s; step 5-melting curve analysis. Steps from…
his system in clp1D or rad24D mutant backgrounds results in cytokinesis failure and the subsequent generation of inviable, multi-nucleate cells. In order to identify other genes with roles in promoting…
22 recurring tumors and develop in females and in males castrated or treated with bicalutamide. Although C4-2 cells divide in androgendepleted medium, androgens still efficiently induced the transcription of a…