These experiments involved relatively few animals but the data seem to indicate that the decreased viability of SirT1-null mice was exacerbated during CR
prior to harvesting for immunohistochemistry with antiLamin A/C and anti-CXCR4. Images were taken using Zeiss Axio Imager.z1 fluorescence microscope at 406magnification at excitation 470 nm for FITC and 551 nm…