Due to the fact Shoc2 lacks any trans-membrane segments, this motif will likely associate with a protein and/or lipids at the periphery of the late endosome membrane
The precipitated portion was analyzed by immuno-blotting (IB) with tRFP and subsequently with Raf-one and HA-antibodies to detect Raf-one and Ras. Cell lysates were immunoblotted with Raf-one antibodies to keep…