The membrane was blocked with nonfat milk, adopted by incubation with key antibody of Bax, Bcl-two and CSE (Abcam, Cambridge, MA) at 4uC overnight
Immediately after 24 hrs, the survival rats were being randomly divided into seven teams: (one) sham team (n = 10) (two) HF group (n = 14) (three) SPRC-handled HF team…